Composite

Part:BBa_K4774013

Designed by: Wong Chak Sum   Group: iGEM23_HKPOLYU   (2023-09-22)


CBD-SsrA+Npu-peptide-Npu 2-CBD2

One-step circularization of EGFR-binding peptide (BBa_K4774000) upon Npu intein (BBa_K4774005 and BBa_K4774007) splicing with higher efficiency and using CBD (Chitin Binding Domain, BBa_K4774002 and BBa_J70627) for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 684
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None